Process


Dna Sequence Analyze

Put your dsDNA or ssDNA sequence in the box below to convert it to or edit it in Inverse, Complement, Double Stranded and Antiparallel Mode

Initial Sequence e.g. 5'- AATGCC -3' Save

Inverse Sequence Sequence in the Opposite Direction (e.g. 3'- CCGTAA -5') Save

Antiparallel Sequence Complementary Sequence in the Same Direction (e.g. 5'- GGCATT -3') Save

Complement Sequence Complementary Sequence in the Opposite Direction (e.g. 3'- TTACGG -5') Save

Double Stranded Sequence Save

Protein Sequence Analyze

Put your Protein sequence in the box below to see its amino acid content

Peptide Sequence e.g. N'- rssiseadli -C' Length: Save

Quantity
Ala Cys Gly Val Leu Ile Met Ser Thr Phe Tyr Trp Asp Glu Asn Gln His Lys Arg Pro
(A) (C) (G) (V) (L) (I) (M) (S) (T) (F) (Y) (W) (D) (E) (N) (Q) (H) (K) (R) (P)
% Frequency In %frequency calculation, unidentified amino acids and stop codons are counted inside total number of amino acids as if they were usual amino acids
Ala Cys Gly Val Leu Ile Met Ser Thr Phe Tyr Trp Asp Glu Asn Gln His Lys Arg Pro
(A) (C) (G) (V) (L) (I) (M) (S) (T) (F) (Y) (W) (D) (E) (N) (Q) (H) (K) (R) (P)

Six Frame Translation

Put your DNA sequence in the box below to translate it in six frames and to align them with original DNA

Frame No: +1 Protein Length: Brutto Formula:
Average Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: +2 Protein Length: Brutto Formula:
Average Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: +3 Protein Length: Brutto Formula:
Average Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: -1 Protein Length: Brutto Formula:
Average Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: -2 Protein Length: Brutto Formula:
Average Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: -3 Protein Length: Brutto Formula:
Average Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Alignment of Original DNA Sequence Relative to Amino Acids Sequences in Different Frames

Amino acids per line

DNA Uppercase Lowercase
+1 frame Uppercase Lowercase Three letter
+2 frame Uppercase Lowercase Three letter
+3 frame Uppercase Lowercase Three letter
-1 frame Uppercase Lowercase Three letter
-2 frame Uppercase Lowercase Three letter
-3 frame Uppercase Lowercase Three letter

Forward Direction: Save

Reverse Direction: Save

Reverse Translation

Put your Protein sequence in the box below to reversely translate it to possible DNA sequences

Get MPC Sequence The information regarding Most Probable Codons(MPC) was obtained from Codon Usage Database (http://www.kazusa.or.jp/codon/) Get FC Sequence Get SC Sequence Get Whole Sequence


Formatting
Polymorphic Letters These are the letters which may correspond to more than one dna base : SWRYMKHBDVN
Disregard Accept
Lowercase
Uppercase
Letters per line :
Block value :
Formatting
Amino acids per line :
Block value :
One Letter Uppercase
One Letter Lowercase
Three Letter
Formatting
Amino acids per line :
Block value :
One Letter Uppercase
One Letter Lowercase
Three Letter
Formatting
Amino acids per line :
Single Letter Type
Codon Type
Calculation
Lineer Circular
Single Stranded
Double Stranded
Average Mw
Monoisotopic Mw
Calculation
Isotop Content
13C 15N D
Monoisotopic Mw
Brutto Formula
Average Mw
Isoelectric Point
рН:
Estimated Charge To find pH specific charge, 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65)