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Input Type
DNA
Protein

Lock Sequence

- Entered DNA Sequence - (Preferences)

-Preferences-
Disregard Polymorphic Letters: SWRYMKHBDVN letters per line with a block value of
Accept Polymorphic Letters: SWRYMKHBDVN Lowercase Uppercase

Initial Sequence e.g. 5'- AATGCC -3' Save

Inverse Sequence Sequence in the Opposite Direction (e.g. 3'- CCGTAA -5') Save

Antiparallel Sequence Complementary Sequence in the Same Direction (e.g. 5'- GGCATT -3') Save

Complement Sequence Complementary Sequence in the Opposite Direction (e.g. 3'- TTACGG -5') Save

Double Stranded Sequence Save

Calculation Part
Average Mw Lineer Single Stranded
Monoisotopic Mw Circular Double Stranded

MW (Da) Amount (ng) Vol. (µl) A260 units Moles (pmol) Conc. (µg/ml)

- Protein sequences translated from entered DNA sequence - (Preferences)

-Preferences-

amino acides in one line with a block value of

Amino Acid Display Type: One Letter Uppercase One Letter Lowercase Three Letter


Frame No: +1 Protein Length: Brutto Formula: Average Mw:
Monoisotopic Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: +2 Protein Length: Brutto Formula: Average Mw:
Monoisotopic Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: +3 Protein Length: Brutto Formula: Average Mw:
Monoisotopic Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: -1 Protein Length: Brutto Formula: Average Mw:
Monoisotopic Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: -2 Protein Length: Brutto Formula: Average Mw:
Monoisotopic Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Frame No: -3 Protein Length: Brutto Formula: Average Mw:
Monoisotopic Mw: Isoelectric Point: To find Isoelectric Points, bisection method was used and only 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65) Save

Calculation Part
Frames Mw(Da) Isotop Content Amount (ng) Moles (picomole) Volume (µL) Conc. (µg/ml) Estimated Charge under
рН: To find pH specific charge, 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65)
+1 13C 15N D
+2 13C 15N D
+3 13C 15N D
-1 13C 15N D
-2 13C 15N D
-3 13C 15N D


Quantity
Ala Cys Gly Val Leu Ile Met Ser Thr Phe Tyr Trp Asp Glu Asn Gln His Lys Arg Pro
Frames(A) (C) (G) (V) (L) (I) (M) (S) (T) (F) (Y) (W) (D) (E) (N) (Q) (H) (K) (R) (P)
+1
+2
+3
-1
-2
-3
% Frequency In %frequency calculation, unidentified amino acids and stop codons are counted inside total number of amino acids as if they were usual amino acides
Ala Cys Gly Val Leu Ile Met Ser Thr Phe Tyr Trp Asp Glu Asn Gln His Lys Arg Pro
Frames(A) (C) (G) (V) (L) (I) (M) (S) (T) (F) (Y) (W) (D) (E) (N) (Q) (H) (K) (R) (P)
+1
+2
+3
-1
-2
-3

Alignment of Original DNA Sequence Relative to Amino Acids Sequences in Different Frames


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amino acides per line


DNA +1 frame +2 frame +3 frame -1 frame -2 frame -3 frame
Uppercase Uppercase Uppercase Uppercase Uppercase Uppercase Uppercase
Lowercase Lowercase Lowercase Lowercase Lowercase Lowercase Lowercase
Three letter Three letter Three letter Three letter Three letter Three letter

Forward Direction: Save

Reverse Direction: Save

- Entered Peptide Sequence - (Preferences)

-Preferences-

amino acides in one line with a block value of

Amino Acid Display Type: One Letter Uppercase One Letter Lowercase Three Letter


Peptide Sequence e.g. N'- rssiseadli -C' Length: Save

Inverse Sequence e.g. C'- ildaesissr -N' Save


Calculation Part
Monoisotopic Mw Brutto Formula Isotop Content
Average Mw Isoelectric Point 13C 15N D

Mw(Da) Amount (ng) Moles (picomole) Volume (µL) Conc.(µg/ml) Estimated Charge under
рН: To find pH specific charge, 7 charged amino acids and NH2+CO2 were taken into account. pKa values are: Cys(8.18), Tyr(10.46), Asp(3.9), Glu(4.07), His(6.04), Lys(10.54), Arg(12.48), NH2(8.2), CO2(3.65)

Quantity
Ala Cys Gly Val Leu Ile Met Ser Thr Phe Tyr Trp Asp Glu Asn Gln His Lys Arg Pro
(A) (C) (G) (V) (L) (I) (M) (S) (T) (F) (Y) (W) (D) (E) (N) (Q) (H) (K) (R) (P)
% Frequency In %frequency calculation, unidentified amino acids and stop codons are counted inside total number of amino acids as if they were usual amino acides
Ala Cys Gly Val Leu Ile Met Ser Thr Phe Tyr Trp Asp Glu Asn Gln His Lys Arg Pro
(A) (C) (G) (V) (L) (I) (M) (S) (T) (F) (Y) (W) (D) (E) (N) (Q) (H) (K) (R) (P)

- DNA Seq.Reversely Translated From Entered Peptide Sequence - (Preferences)

-Preferences-

: amino acides per line

Reverse Translation Display Type: Single Letter Type Codon Type

Get MPC Sequence The information regarding Most Probable Codons(MPC) was obtained from Codon Usage Database (http://www.kazusa.or.jp/codon/) Get FC Sequence Get SC Sequence Get Whole Sequence




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author:erdalyilmaz@itu.edu.tr